Coding
Part:BBa_K4790071:Design
Designed by: Yi Zheng Group: iGEM23_NNU-CHINA (2023-09-28)
ASR6(node 112)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 281
Illegal PstI site found at 292
Illegal PstI site found at 418 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 268
Illegal PstI site found at 281
Illegal PstI site found at 292
Illegal PstI site found at 418 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 288
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 281
Illegal PstI site found at 292
Illegal PstI site found at 418 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 281
Illegal PstI site found at 292
Illegal PstI site found at 418
Illegal NgoMIV site found at 264 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Firstly, we use profile-HMM algorithm instead of BLAST local alignment algorithm. To obtain PETase-related protein sequences from all species, Pfam entries and HMMER web server were used to search Swiss-prot and NCBI public databases. Then we set up a database of PETase protein sequences by selecting relatively strict sequences according to e-value, and manually adding sequences identified by homology. Finally, the phylogenetic tree was established through the ML algorithm of fireprot server, and several ancestor sequences of PETase were obtained according to the evolved nodes, among which the most primitive sequence was node 112.
Source
It does not come from any genomic sequence.